IgG from Human Plasma

One step purification of IgG from Human Plasma with Simul-A

	Lanes Markers 0 ul sample IgG post dialfiltration 40 ul sample IgG post diafiltration 20 ul dilute crude plasma 40 ul dilute crude plasma Reduced sample: 40 ul lane 2 + b-ME

A more conventional procedure avoids Cohn Fraction I contaminants and uses Cohn Fraction II, III paste solution as starting material. Human IgG is routinely obtained in about 98% purity in yields greater than 75% to 80%. This is a considerable improvement over reported commercial manufacturing processes involving a combination of several chromatographic steps beyond Cohn II, III paste solution.