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As the trend of rapid expansion in use of biologicals for therapeutic applications continues, the demands for more efficient downstream processing grow in parallel. To efficiently and profitably handle the ever-larger volumes of biological broths, novel approaches in separation methodologies, as well as in new, more effective and more specific materials, are needed. APL's advanced Orbicell® bead technology enables biopharmaceutical companies to realize greater profits by reducing manufacturing costs resulting from both, shorter processing cycles and increased product yields, with no apparent penalties in the final product purity. Compared to the current classic column systems, APL's new Orbicell® Technology used in downstream processing enables companies to reach higher productivity levels and increase their bottom lines.
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Old Column Systems |
New APL Biopurification
Technologies
Orbicell© Bioresins
Used in a Continuous Flow System |
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The geometry of a column - e.g. diameter and bed height - limits the amount of biomaterial to be purified
The only variable is the bed height. If you increase the bed height you slow down the flow through the column. |
In contrast to column chromatography the amount of Orbicell® resin to be used in a purification cycle is solely determined by the amount of product to be purified.
The speed of a cycle is determined by the ratio of the surface of the microfilter to the amount of beads used in a particular application. For example, if you need to double the speed of a cycle, double the number of filtering modules and and increase the pumping rate accordingly. |
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Resins with porous particles trap contaminants in their matrix, extending the wash and elution cycles. |
Non-porous Orbicell® bioresins do not trap impurities in their interior allowing for faster and more complete wash and elution cycles. |
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Columns sometimes exhibit channeling that leads to impure fractions. Also, they may exhibit dead spots, resulting in an entrapment of contaminants. |
A non-porous bed suspension in a flow system does not accumulate or trap contaminants during purification. |
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